窦硕星

简介


男,1966年3月生。1985年毕业于山东大学光学系;1988年7月于山东大学晶体材料研究所获硕士学位;1992年11月于法国居里大学(巴黎六大)获理学博士学位。曾先后在法国、德国和韩国进行高访研究。现任中国科学院物理研究所研究员、博士生导师。

主要研究方向


生物物理,主要是DNA解旋酶等生物马达蛋白的运动机理。

过去的主要工作及获得的成果


1985年至2001年,主要从事非线性光学等方面的研究工作。2002年开始从事DNA与蛋白质相互作用以及各种生物分子马达工作运动机理的研究。曾参加或主持多项国家自然科学基金面上和重点项目、863及攀登计划项目、科学院知识创新工程方向性项目。先后发表SCI论文100余篇,其中生物物理方面论文60余篇。

代表性论文及专利


1. J. You, H. Li, X.-M. Lu, W. Li, P.-Y. Wang, S. X. Dou, and X.-G. Xi, “Effects of monovalent cations on folding kinetics of G-quadruplexes.” Bioscience Reports 37, BSR20170771 (2017).
2. W. X. Lin, J. B. Ma, D. G. Nong, C. H. Xu, B. Zhang, J. H. Li, Q. Jia, S. X. Dou, F. F. Ye, X. G. Xi, Y. Lu, and M. Li, “Helicase stepping investigated with one-nucleotide resolution fluorescence resonance energy transfer.” Phys. Rev. Lett. 119, 138102 (2017).
3. W.-Q. Wu, X.-M. Hou, B. Zhang, P. Fossé, B. René, O. Mauffret, M. Li, S. X. Dou, and X.-G. Xi, “Single-molecule studies reveal reciprocating of WRN helicase core along ssDNA during DNA unwinding.” Scientific Reports 7, 43954 (2017).
4. W.-F. Chen, Y.-X. Dai, X.-L. Duan, N.-N. Liu, W. Shi, N. Li, M. Li, S. X. Dou, Y.-H. Dong, S. Rety, and X.-G. Xi, "Crystal structures of the BsPif1 helicase reveal that a major movement of the 2B SH3 domain is required for DNA unwinding." Nucleic Acids Res. 44, 2949-2961 (2016).
5. B. Zhang, W.-Q. Wu, N.-N. Liu, X.-L. Duan, M. Li, S. X. Dou, X.-M. Hou, and X.-G. Xi, “G-quadruplex and G-rich sequence stimulate Pif1p-catalyzed downstream duplex DNA unwinding through reducing waiting time at ss/dsDNA junction.” Nucleic Acids Res. 44, 8385-8394 (2016).
6. J.-H. Li, W.-X. Lin, B. Zhang, D.-G. Nong, H.-P. Ju, J.-B. Ma, C.-H. Xu, F.-F. Ye, X. G. Xi, M. Li, Y. Lu, and S. X. Dou, “Pif1 is a force-regulated helicase.” Nucleic Acids Res. 44, 4330-4339 (2016).
7. H.-P. Ju, Y.-Z. Wang, J. You, X.-M. Hou, X.-G. Xi, S. X. Dou, W. Li, and P.-Y. Wang, “Folding kinetics of single human telomeric G-quadruplex affected by cisplatin.” ACS Omega 1, 244-250 (2016).
8. Y.-W. Zhang, D.-G. Nong, S. X. Dou, W. Li, Y. Yan, X.-G. Xi, C.-H. Xu, and M. Li, “Iterative homology checking and non-uniform stepping during RecA-mediated strand exchange.” Biochem. Biophys. Res. Commun. 478, 1153e1157 (2016).
9. N.-N. Liu, X.-L. Duan, X. Ai, Y.-T. Yang, M. Li, S. X. Dou, S. Rety, E. Deprez, and X.-G. Xi, “The Bacteroides sp. 3_1_23 Pif1 protein is a multifunctional helicase.” Nucleic Acids Res. 43, 8942-8954 (2015).
10. Y. R. Liu, P. Xie, P. Y. Wang, M. Li, H. Li, W. Li, and S. X. Dou, “A model for chromosome organization during the cell cycle in live E. coli.” Scientific Reports 5, 17133 (2015).
11. W.-Q. Wu, X.-M. Hou, M. Li, S. X. Dou, and X.-G. Xi, “BLM unfolds G-quadruplexes in different structural environments through different mechanisms.” Nucleic Acids Res. 43, 4614-4626 (2015).
12. X.-L. Duan, N.-N. Liu, Y.-T. Yang, H.-H. Li, M. Li, S. X. Dou, and X.-G. Xi, “G-quadruplexes significantly stimulate Pif1 helicase-catalyzed duplex DNA unwinding.” J. Biol. Chem. 290, 7722-7735 (2015).
13. S. Wang, W. Qin, J.-H. Li, Y. Lu, K.-Y. Lu, D.-G. Nong, S. X. Dou, C.-H. Xu, X,-G. Xi, and M. Li, “Unwinding forward and sliding back: an intermittent unwinding mode of the BLM helicase.” Nucleic Acids Res. 43, 3736-3746 (2015).1
4. X.-M. Hou, W.-Q. Wu, X.-L. Duan, N.-N. Liu, H.-H. Li, J. Fu, S. X. Dou, M. Li, and X.-G. Xi, “Molecular mechanism of G-quadruplex unwinding helicase: sequential and repetitive unfolding of G-quadruplex by Pif1 helicase.” Biochem. J. 466, 189-199 (2015).

15. H. Li, S. X. Dou, Y.-R. Liu, W. Li, P. Xie, W.-C. Wang, and P.-Y. Wang. “Mapping intracellular diffusion distribution using single quantum dot tracking: compartmentalized diffusion defined by endoplasmic reticulum.” J. Am. Chem. Soc. 137, 436-444 (2015).
16. H.-Y. Zhang, L.-Y. Zhang, W.-C. Wang, P. Xie, S. X. Dou, C. Ji, W. Li, Y.-R. Liu, and P.-Y. Wang. “Oxaliplatin and its enantiomer induce different condensation dynamics of single DNA molecules.” PLOS ONE 8, e71556 (2013).
17. Y.-R. Liu, S.-X. Dou, C. Ji, W.-C. Wang, P. Xie, H.-Y. Zhang, and P.-Y. Wang. “Transplatin enhances effect of cisplatin on both single DNA molecules and live tumor cells.” Archives Biochem. Biophys. 536, 12-24 (2013).
18. H. Li, Z. W. Duan, P. Xie, Y. R. Liu, W. C. Wang, S. X. Dou, and Y. P. Wang. “Effects of paclitaxel on EGFR endocytic trafficking revealed using quantum dot tracking in single cells.” PLOS ONE 7, e45465 (2012).
19. Y.-N. Xu, N. Bazeille, X.-Y. Ding, X.-M. Lu, P.-Y. Wang, E. Bugnard, V. Grondin, S. X. Dou, and X. G Xi, “Multimeric BLM is dissociated upon ATP hydrolysis and functions as monomers in resolving DNA structures.” Nucleic Acids Res. 40, 9802-9814 (2012).
20. W. Li, Z. Q. Sun, P. Xie, S. X. Dou, W. C. Wang, and P. Y. Wang, “Elastic response and length change of single DNA molecules induced by a combination of cisplatin and transplatin.” Phys. Rev. E 85, 021918 (2012).
21. S. X. Dou and X. G. Xi, “Fluorometric assays for characterizing DNA helicases.” Methods 51, 295-302 (2010).
22. B.-Y. Pan, S. X. Dou, Y. Yang, Y.-N. Xu, E. Bugnard, X.-Y. Ding, L. Zhang, P.-Y. Wang, M. Li, and X. G. Xi, “Mutual inhibition of RecQ molecules in DNA unwinding.” J. Biol. Chem. 285, 15884-15893 (2010).
23. Y. Yang, S. X. Dou, Y.-N. Xu, P.-Y. Wang, P. Rigolet, H.-Q. Xu, and X. G. Xi, “Kinetic mechanism of DNA unwinding by the BLM helicase core and molecular basis for its low processivity.” Biochemistry 49, 656-668 (2010).
24. X.-M. Hou, X.-H. Zhang, K.-J. Wei, C. Ji, S. X. Dou, W.-C. Wang, M. Li, P.-Y. Wang, “Cisplatin induces loop structures and condensation of single DNA molecules.” Nucleic Acids Res. 37, 1400-1410 (2009).
25. B.-Y. Pan, L. Zhang, S. X. Dou, and P.-Y. Wang, “Forces-induced pinpoint denaturation of short DNA.” Biochem. Biophys. Res. Commun. 388, 137-140 (2009).
26. X.-M. Hou, W. Li, S. X. Dou, L.-Y. Zhang, P. Xie, W.-C Wang, P.-Y. Wang, “Formation of DNA toroids inside confined droplets adsorbed on mica surfaces.” Phys. Rev. E 79, 051912 (2009).
27. B. Sun, K.-J. Wei, B. Zhang, X.-H. Zhang, S. X. Dou, M. Li, X. G. Xi, “Impediment of E. coli UvrD by DNA-destabilizing force reveals a strained-inchworm mechanism of DNA unwinding.” EMBO J. 27, 3279-3287 (2008).
28. Y. Yang, S. X. Dou, H. Ren, P. Y. Wang, X. D. Zhang, M. Qian, B. Y. Pan, X. G. Xi, “Evidence for a functional dimeric form of the PcrA helicase in DNA unwinding.” Nucleic Acids Res. 36, 1976-1989 (2008).
29. H. Ren, S. X. Dou, X.-D. Zhang, P.-Y. Wang, R. Kanagaraj, J.-L. Liu, P. Janscak, J.-S. Hu, X. G. Xi, “The zinc-binding motif of human RECQ5β suppresses the intrinsic strand-annealing activity of its DExH helicase domain and is essential for the helicase activity of the enzyme.” Biochem. J. 412, 425-433 (2008).
30. H. Ren, S. X. Dou, P. Rigolet, Y. Yang, P.-Y. Wang, M. Amor-Gueret, X. G. Xi, “The arginine finger of the Bloom syndrome protein: its structural organization and its role in energy coupling.” Nucleic Acids Res. 35, 6029-6041 (2007).
31. R.-B. Guo, P. Rigolet, H. Ren, B. Zhang, X.-D. Zhang, S.-X. Dou, P.-Y. Wang, M. Amor-Gueret, X. G. Xi, “Structural and functional analyses of disease-causing missense mutations in Bloom syndrome protein.” Nucleic Acids Res. 35, 6297-6310 (2007).
32. W. Li, S. X. Dou, P. Xie, P. Y. Wang, “Brownian dynamics simulation of the effect of histone modification on nucleosome structure.” Phys. Rev. E 75, 051915 (2007).
33. W. Li, S. X. Dou, P. Xie, P. Y. Wang, “Browinan dynamics simulation of directional sliding of histone octamers caused by DNA bending.” Phys. Rev. E 73, 051909 (2006).
34. X. D. Zhang, S. X. Dou, P. Xie, J. S. Hu, P. Y. Wang, X. G. Xi, “E. coli RecQ is a rapid, efficient and monomeric helicase.” J. Biol. Chem. 281, 12655-12663 (2006).
35. X. D. Zhang, S. X. Dou, P. Xie, P. Y. Wang, X. G. Xi, “RecQ helicase-catalyzed DNA unwinding detected by fluorescence resonance energy transfer.” Acta Biochimi. Biophys. Sin. 37, 593-600 (2005).
36. J. L. Liu, P. Rigolet, S. X. Dou, P. Y. Wang, X. G. Xi, “The Zinc finger motif of Escherichia coli RecQ is implicated in both DNA binding and protein folding.” J. Biol. Chem. 279, 42794-42802 (2004).
37. S. X. Dou, P. Y. Wang, H. Q. Xu, X. G. Xi, “The DNA binding properties of the Escherichia coli RecQ helicase.” J. Biol. Chem. 279, 6354-6363 (2004).
38. Y. Y. Liu, P. Y. Wang, S. X. Dou, W. C. Wang, P. Xie, H. W. Yin, X. D. Zhang, X. G. Xi, “Ionic effect on combing of single DNA molecules and observation of their force-induced melting by fluorescence microscopy.” J. Chem. Phys. 121, 4302-4309 (2004).

目前的研究课题及展望


通过应用单分子荧光显微技术、磁镊、AFM等现代光学、物理方法,以及快速反应动力学等传统的生物物理及化学方法,研究生物体内各种马达蛋白(如DNA解旋酶、RNA解旋酶、DNA聚合酶等)的生物学特性、功能及其能量转换机制,为探索疾病治疗的新途径打下基础。

培养研究生情况


在读博士生2名,参与培养毕业博士生9名。欢迎来自生物、物理、化学专业的学生报考。

电话:

010-82649484

Email

sxdou@iphy.ac.cn