窦硕星

简介:

男,1966年3月生。1985年毕业于山东大学光学系;1988年7月于山东大学晶体材料研究所获硕士学位;1992年11月于法国居里大学(巴黎六大)获理学博士学位。曾先后在法国、德国和韩国进行高访研究。现任中国科学院物理研究所研究员、博士生导师。

主要研究方向:

生物物理,主要是DNA解旋酶等生物马达蛋白的运动机理。

过去的主要工作及获得的成果:

1985年至2001年,主要从事非线性光学等方面的研究工作。2002年开始从事DNA与蛋白质相互作用以及各种生物分子马达工作运动机理的研究。曾参加或主持多项国家自然科学基金面上、重点项目、863计划及攀登计划项目、科学院知识创新工程方向性项目。在国内外重要学术刊物上先后发表SCI论文100多篇,其中生物物理方面论文50余篇(杂志包括 Nucleic Acids Res、J Am Chem Soc、J Biol Chem、EMBO J、Biochem J、Phys Rev E等)。

代表性论文及专利:

1. J.-H. Li, W.-X. Lin, B. Zhang, D.-G. Nong, H.-P. Ju, J.-B. Ma, C.-H. Xu, F.-F. Ye, X. G. Xi, M. Li, Y. Lu, and S. X. Dou, “Pif1 is a force-regulated helicase.” Nucleic Acids Res. 44, 4330–4339 (2016).
2. W.-F. Chen, Y.-X. Dai, X.-L. Duan, N.-N. Liu, W. Shi, N. Li, M. Li, S. X. Dou, Y.-H. Dong, S. Rety, and X.-G. Xi, "Crystal structures of the BsPif1 helicase reveal that a major movement of the 2B SH3 domain is required for DNA unwinding." Nucleic Acids Res. 44, 2949–2961 (2016).
3. N.-N. Liu, X.-L. Duan, X. Ai, Y.-T. Yang, M. Li, S. X. Dou, S. Rety, E. Deprez, and X.-G. Xi, “The Bacteroides sp. 3 1 23 Pif1 protein is a multifunctional helicase.” Nucleic Acids Res. 43, 8942–8954 (2015).
4. W.-Q. Wu, X.-M. Hou, M. Li, S. X. Dou, and X.-G. Xi, “BLM unfolds G-quadruplexes in different structural environments through different mechanisms.” Nucleic Acids Res. 43, 4614–4626 (2015).
5. S. Wang, W. Qin, J.-H. Li, Y. Lu, K.-Y. Lu, D.-G. Nong, S. X. Dou, C.-H. Xu, X,-G. Xi, and M. Li, “Unwinding forward and sliding back: an intermittent unwinding mode of the BLM helicase.” Nucleic Acids Res. 43, 3736–3746 (2015).
6. X.-L. Duan, N.-N. Liu, Y.-T. Yang, H.-H. Li, M. Li, S. X. Dou, and X.-G. Xi, “G-quadruplexes significantly stimulate Pif1 helicase-catalyzed duplex DNA unwinding.” J. Biol. Chem. 290, 7722–7735 (2015).
7. H. Li, S. X. Dou, Y.-R. Liu, W. Li, P. Xie, W.-C. Wang, and P.-Y. Wang. “Mapping Intracellular Diffusion Distribution Using Single Quantum Dot Tracking: Compartmentalized Diffusion Defined by Endoplasmic Reticulum.” J. Am. Chem. Soc. 137, 436–444 (2015).
8.  X.-M. Hou, W.-Q. Wu, X.-L. Duan, N.-N. Liu, H.-H. Li, J. Fu, S. X. Dou, M. Li, and X.-G. Xi, “Molecular mechanism of G-quadruplex unwinding helicase: sequential and repetitive unfolding of G-quadruplex by Pif1 helicase.” Biochem. J. 466, 189–199 (2015).
9.  Y.-N. Xu, N. Bazeille, X.-Y. Ding, X.-M. Lu, P.-Y. Wang, E. Bugnard, V. Grondin, S. X. Dou, and X. G Xi, “Multimeric BLM is dissociated upon ATP hydrolysis and functions as monomers in resolving DNA structures.” Nucleic Acids Res. 40, 9802-9814 (2012).
10.  W. Li, Z. Q. Sun, P. Xie, S. X. Dou, W. C. Wang, and P. Y. Wang, “Elastic response and length change of single DNA molecules induced by a combination of cisplatin and transplatin.” Phys. Rev. E 85, 021918 (2012).
11.  S. X. Dou and X. G. Xi, “Fluorometric assays for characterizing DNA helicases.” Methods 51, 295-302 (2010).
12.  B.-Y. Pan, S. X. Dou, Y. Yang, Y.-N. Xu, E. Bugnard, X.-Y. Ding, L. Zhang, P.-Y. Wang, M. Li, and X. G. Xi, “Mutual inhibition of RecQ molecules in DNA unwinding.” J. Biol. Chem. 285, 15884-15893 (2010).
13.  Y. Yang, S. X. Dou, Y.-N. Xu, P.-Y. Wang, P. Rigolet, H.-Q. Xu, and X. G. Xi, “Kinetic mechanism of DNA unwinding by the BLM helicase core and molecular basis for its low processivity.” Biochemistry 49, 656-668 (2010).
14.  X.-M. Hou, X.-H. Zhang, K.-J. Wei, C. Ji, S. X. Dou, W.-C. Wang, M. Li, P.-Y. Wang, “Cisplatin induces loop structures and condensation of single DNA molecules.” Nucleic Acids Res. 37, 1400-1410 (2009).
15.  B. Sun, K.-J. Wei, B. Zhang, X.-H. Zhang, S. X. Dou, M. Li, X. G. Xi, “Impediment of E. coli UvrD by DNA-destabilizing force reveals a strained-inchworm mechanism of DNA unwinding.” EMBO J. 27, 3279-3287 (2008).
16.  Y. Yang, S. X. Dou, H. Ren, P. Y. Wang, X. D. Zhang, M. Qian, B. Y. Pan, X. G. Xi, “Evidence for a functional dimeric form of the PcrA helicase in DNA unwinding.” Nucleic Acids Res. 36, 1976-1989 (2008).
17.  H. Ren, S. X. Dou, X.-D. Zhang, P.-Y. Wang, R. Kanagaraj, J.-L. Liu, P. Janscak, J.-S. Hu, X.G. Xi, “The zinc-binding motif of human RECQ5β suppresses the intrinsic strand-annealing activity of its DExH helicase domain and is essential for the helicase activity of the enzyme.” Biochem. J. 412, 425-433 (2008).
18.  H. Ren, S. X. Dou, P. Rigolet, Y. Yang, P.-Y. Wang, M. Amor-Gueret, X. G. Xi, “The arginine finger of the Bloom syndrome protein: its structural organization and its role in energy coupling.” Nucleic Acids Res. 35, 6029-6041 (2007).
19.  R.-B. Guo, P. Rigolet, H. Ren, B. Zhang, X.-D. Zhang, S.-X. Dou, P.-Y. Wang, M. Amor-Gueret, X. G. Xi, “Structural and functional analyses of disease-causing missense mutations in Bloom syndrome protein.” Nucleic Acids Res. 35, 6297-6310 (2007).
20.  W. Li, S. X. Dou, P. Xie, P. Y. Wang, “Brownian dynamics simulation of the effect of histone modification on nucleosome structure.” Phys. Rev. E 75, 051915 (2007).
21.  W. Li, S. X. Dou, P. Xie, P. Y. Wang, “Browinan dynamics simulation of directional sliding of histone octamers caused by DNA bending.” Phys. Rev. E 73, 051909 (2006).
22.  X. D. Zhang, S. X. Dou, P. Xie, J. S. Hu, P. Y. Wang, X. G. Xi, “E. coli RecQ is a rapid, efficient and monomeric helicase.” J. Biol. Chem. 281, 12655-12663 (2006).
23.  J. L. Liu, P. Rigolet, S. X. Dou, P. Y. Wang, X. G. Xi, “The Zinc finger motif of Escherichia coli RecQ is implicated in both DNA binding and protein folding.” J. Biol. Chem. 279, 42794-42802 (2004).

目前的研究课题及展望:

正在或拟开展的研究主要是通过应用单分子荧光显微技术、光镊、磁镊、AFM等现代光学、物理方法,以及传统的生物物理及化学方法,理论与实验相结合,研究生物体内各种马达蛋白(如DNA解旋酶、RNA解旋酶、端粒酶以及主要负责运输功能的驱动蛋白、动力蛋白等)的生物学特性及其能量转换机制,为了解生物细胞内的物质能量传输和功能调控、探索疾病治疗的新途径打下基础。

培养研究生情况:

在读博士生2名,参与培养博士研究生7名(已毕业5名)。欢迎来自生物、物理、化学专业的学生报考。

电话:

010-82649484

Email:

sxdou@iphy.ac.cn