陆颖
简介：
2004年于北京工业大学获得学士学位。
2010年于中国科学院物理研究所获理学博士学位（导师：李明研究员）。
2010年7月至今中国科学院物理研究所软物质物理重点实验室工作。
2020年获国家自然科学基金委优秀青年基金。
主要研究方向：
聚焦于物理学与生命科学的交叉领域，致力于利用超高精度单分子荧光技术，在分子水平上揭示生命过程的运行机制。目前主要研究方向包括：
生物膜系统的复杂动力学：在单分子水平上，表征从离体到在体环境中生物膜及相关膜蛋白的随机动力学、输运过程及其相变行为。
人工细胞膜的形变及输运：探索膜蛋白作为“分子机器”，介导人工细胞膜的形变，并调控其跨膜输运的统计物理规律。
过去的主要工作及获得的成果：
1.发展了精度达到亚纳米的表面诱导荧光衰减方法，一举突破膜蛋白位置测量的技术瓶颈，可实时测量膜蛋白插膜的动力学。运用该方法，解析了多肽的跨膜过程。
2.发展了基于囊泡的荧光淬灭方法，此技术可以用于更为精确地研究接近真实生命过程的蛋白与膜相互作用的动态过程。
3.发展了DNA弓方法，用于提高单分子荧光共振能量转移的精度，应用该方法研究核酸酶，可以精确测量单个水解能量所驱动的分子马达在核酸上的运动，极大提高分子马达的研究能力。
代表性论文及专利：
2025年
Ye Yuan, Hao Wang, Hang Fu, Chenguang Yang, Zhao Lin, Chunhua Xu, Shuxin Hu,
Tongsheng Chen, Qi Jia, Ming Li, and Ying Lu. Mismatched Nucleotide Rejections at the Open and Ajar States of DNA Polymerase I Ensure High Fidelity. J. Am. Chem. Soc., 2025, 147: 46094-46100.
Jiaxuan Li, Chenguang Yang, Ruihan Dong, Juan Francisco Bada Juarez, Lei Wang, Maximilian Emanuel Wettstein, Dali Wang, Chan Cao, Ying Lu, Chen Song. Mechanism-Driven Screening of Membrane-Targeting and Pore-Forming Antimicrobial Peptides. Adv.Sci., 2025, e16470.
Wang, Lin; Zhang, Haonan; Jia, Qi; Li, Wenyan; Yang, Chenguang; Ma, Lijuan; Li, Ming; Lu, Ying; Zhu, Hongtao; Zhu, Ping. Cryo-EM structures reveal the acetylation process of piccolo NuA4. Proc. Natl. Acad. Sci. USA, 2025, 122(12):e2414490122.
Huang, Shujing; Ma, Dongfei; Yu, Caiting; Li, Jing; Tu, Xinyu; Huang, Zi; Qi, Yuanbo; Ou, Junying; Feng, Jianxiong; Yu, Bing; Cao, Yulu; Yue, Jiaxing; Hu, Junjie; Li, Ming; Lu, Ying; Yan, Liming; Gao, Song. A Special Latch in Yeast Mitofusin Guarantees Mitochondrial Fusion by Stabilizing Self-Assembly. Nat. Commun., 2025, 16: 9644.
Wang, Hao; Fu, Hang; Yuan, Ye; Yang, Chenguang; Lin, Zhao; Chen, Tongsheng; Xu, Chunhua; Hu, Shuxin; Li, Ming; Lu, Ying. Photon-by-Photon Fluorescence Lifetime Analysis of within-Burst State Transitions of Single Proteins in Lipid Membranes. J. Am. Chem. Soc., 2025, 147: 28552-28557.
Qiu, Jie; Ma, Dongfei; You, Xin; Jia, Qi; Hu, Shuxin; Xu, Cheng; Cao, Binglong; Yang, Kai; Han, Weijing; Lu, Ying; Yuan, Bing; Li, Ming. Protonation-Regulated Membrane-Insertion Dynamics of pH Low-Insertion Peptide: Metastable Molecular Conformations and Their Transitions. ACS Nano, 2025, 19: 15685-15697.
2024年
Ma, Dongfei; Zhang, Shenqing; Xu, Siyao; Huang, Zi; Tao, Yuanxiao; Chen, Feiyang; Zhang, Shengnan; Li, Dan; Chen, Tongsheng; Liu, Cong; Li, Ming; Lu, Ying. Self-limiting multimerization of α-synuclein on membrane and its implication in Parkinson’s diseases. Sci. Adv., 2024, 10(41).
Li, Na; Ma, Jianbing; Fu, Hang; Yang, Zhiwei; Xu, Chunhua; Li, Haihong; Zhao, Yimin; Zhao, Yizhen; Chen, Shuyu; Gou, Lu; Zhang, Xinghua; Zhang, Shengli; Li, Ming; Hou, Ximiao; Zhang, Lei; Lu, Ying. Four Parallel Pathways in T4 Ligase-catalyzed Repair of Nicked DNA with Diverse Bending Angles. Adv.Sci., 2024, 11(25): 2401150.
Xu, Chunhua; Lu, Yue; Wu, Yichao; Yuan, Shuaikang; Ma, Jianbing; Fu, Hang; Wang, Hao; Wang, Libang; Zhang, Hao; Yu, Xuan; Tao, Wei; Liu, Chang; Hu, Shuxin; Peng, Yi; Li, Wenfei; Li, Yunliang; Lu, Ying; Li, Ming. Sodium Ion-induced Structural Transition on the Surface of a DNA-interacting Protein. Adv. Sci., 2024, 11: 2401838.
2023年
Yang, Chenguang; He, Xiaolong; Wang, Hao; Lin, Zhao; Hou, Wenqing; Lu, Ying; Hu, Shuxin; Li, Ming. Single-Molecule Monitoring of Membrane Association of the Necroptosis Executioner MLKL with Discernible Anchoring and Insertion Dynamics. Nano Lett., 2023, 23(2): 4770-4777.
Wang, Mengxue; Li, Jinghua; Wang, Yong; Fu, Hang; Qiu, Haoning; Li, Yanying; Li, Ming; Lu, Ying; Fu, Yu Vincent. Single-molecule study reveals Hmo1, not Hho1, promotes chromatin assembly in budding yeast. mBio, 2023,14(4): e0099323.
2022年
Li, Jinghua; Ma, Jianbing; Kumar, Vikash; Fu, Hang; Xu, Chunhua; Wang, Shuang; Jia, Qi; Fan, Qinkai; Xi, Xuguang; Li, Ming; Liu, Haiguang; Lu, Ying. Identification of flexible Pif1–DNA interactions and their impacts on enzymatic activities. Nucleic Acids Res., 2022, 50(12): 7002-7012.
Lu, Ying; Ma, Jianbing; Li, Ming. Chapter 3, Single-Molecule Biosensing by Fluorescence Resonance Energy Transfer. Jenny Stanford Publishing, 2022.
Ma, Jianbing; Xu, Chunhua; Li, Jinghua; Hou, Ximiao; Da, Lintai; Jia, Qi; Huang, Xingyuan; Yu, Jin; Xi, Xuguang; Lu, Ying; Li, Ming. RQC helical hairpin in Bloom's syndrome helicase regulates DNA unwinding by dynamically intercepting nascent nucleotides. iScience, 2022, 25(1): 103606.
Jiang, Xin; Yang, Chenguang; Qiu, Jie; Ma, Dongfei; Xu, Chen; Hu, Shuxin; Han, Weijing; Yuan, Bing; Lu, Ying. Nanomolar LL-37 induces permeability of a biomimetic mitochondrial membrane. Nanoscale, 2022, 14: 15654-15660.
Wang, Shuang; Lu, Ying; Li, Ming. Single-molecular methodologies for the physical biology of protein machines. Chinese Physics B, 2022, 31: 28702.
2021年
Fan, Qihui; Zheng, Yu; Wang, Xiaochen; Xie, Ruipei; Ding, Yu; Wang, Boyi; Yu, Xiaoyu; Lu, Ying; Liu, Liyu; Li, Yunliang; Li, Ming; Zhao, Yuanjin; Jiao, Yang; Ye, Fangfu. Dynamically Re-Organized Collagen Fiber Bundles Transmit Mechanical Signals and Induce Strongly Correlated Cell Migration and Self-Organization. Angew. Chem. Int. Ed., 2021, 60(21): 11858-11867.
Hou, Wenqing; Ma, Dongfei; He, Xiaolong; Han, Weijing; Ma, Jianbing; Wang, Hao; Xu, Chunhua; Xie, Ruipei; Fan, Qihui; Ye, Fangfu; Hu, Shuxin; Li, Ming; Lu, Ying. Subnanometer-Precision Measurements of Transmembrane Motions of Biomolecules in Plasma Membranes Using Quenchers in Extracellular Environment. Nano Lett., 2021, 21(1): 485-491.
2020年
Ma, Jianbing; Chen, Ze; Xu, Chunhua; Huang, Xingyuan; Jia, Qi; Zou, Zhenyu; Mi, Chenyang; Ma, Dongfei; Lu, Ying; Zhang, Huidong; Li, Ming. Dynamic structural insights into the molecular mechanism of DNA unwinding by the bacteriophage T7 helicase. Nucleic Acids Res., 2020, 48(6): 3156-3164.
Huang, Xingyuan; Lu, Ying; Wang, Shuang; Sui, Mingyu; Li, Jinghua; Ma, Jianbing; Ma, Dongfei; Jia, Qi; Hu, Shuxin; Xu, Chunhua; Li, Ming. Mismatch sensing by nucleofilament deciphers mechanism of RecA-mediated homologous recombination. Proc. Natl. Acad. Sci. USA, 2020, 117(34): 20549-20554.
2020年以前
Zhang, Qian; Wen, Fengcai; Zhang, Siqi; Jin, Jiachuan; Bi, Lulu; Lu, Ying; Li, Ming; Xi, Xuguang; Huang, Xingxu; Shen, Bin; Sun, Bo. The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation. Sci. Adv., 2019, 5(11): eaaw9807.
Ma, Dongfei; Xu, Chunhua; Hou, Wenqing; Zhao, Chunyu; Ma, Jianbing; Huang, Xingyuan; Jia, Qi; Ma, Lu; Diao, Jiajie; Liu, Cong; Li, Ming; Lu, Ying. Detecting Single-Molecule Dynamics on Lipid Membranes with Quenchers-in-a-Liposome FRET. Angew. Chem. Int. Ed., 2019, 58(17): 5577-5581.
Li, Meijing; Xia, Xian; Tian, Yuanyuan; Jia, Qi; Liu, Xiaoyu; Lu, Ying; Li, Ming; Li, Xueming; Chen, Zhucheng. Mechanism of DNA translocation underlying chromatin remodelling by Snf2. Nature, 2019, 567(7748): 409-413.
Lin, Wenxia; Ma, Jianbing; Nong, Daguan; Xu, Chunhua; Zhang, Bo; Li, Jinghua; Jia, Qi; Dou, Shuoxing; Ye, Fangfu; Xi, Xuguang; Lu, Ying; Li, Ming. Helicase Stepping Investigated with One-Nucleotide Resolution Fluorescence Resonance Energy Transfer. Phys. Rev. Lett., 2017, 119(13): 138102.
Li, Ying; Qian, Zhenyu; Ma, Li; Hu, Shuxin; Nong, Daguan; Xu, Chunhua; Ye, Fangfu; Lu, Ying; Wei, Guanghong; Li, Ming. Single-molecule visualization of dynamic transitions of pore-forming peptides among multiple transmembrane positions. Nat. Commun., 2016, 7: 12906.
Li, Jinghua; Lin, Wenxia; Zhang, Bo; Nong, Daguan; Ju, Haipeng; Ma, Jianbing; Xu, Chunhua; Ye, Fangfu; Xi, Xuguang; Li, Ming; Lu, Ying; Dou, Shuoxing. Pif1 is a force-regulated helicase. Nucleic Acids Res., 2016, 44(9): 4330-4339.
目前的研究课题及展望：
1.离体和在体生物膜以及相关膜蛋白的动力学过程。
2.膜蛋白介导人工细胞膜可控形貌重构与跨膜输运。
培养研究生情况：
拟每年招收研究生（硕博连读生或博士生）1-3名。
每年招聘博士后1-3名，具有物理学、细胞生物学、分子生物学背景均可。
其他联系方式：
010-82648122
电话：
010-82648122
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